The [KO Validated] SUZ12 Antibody (CAB7786) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting SUZ12 in various biological samples, making it ideal for use in techniques such as Western blotting.SUZ12 is essential for the function of PRC2, which plays a critical role in gene regulation by modifying chromatin structure. Dysregulation of SUZ12 has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SUZ12 Antibody
SKU:
CAB7786
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CHET9, IMMAS, JJAZ1, 12
Positive Sample:
HeLa, BT-474, MCF-7, 293T
Cellular Localization:
Nucleus.
Calculated MW:
83kDa
Observed MW:
83kDa
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
Purification Method
Affinity purification
Gene ID
23512
RRID
AB_2863561
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] SUZ12 Rabbit pAb (CAB7786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and SUZ12 knockout (KO) HeLa cells, using [KO Validated] SUZ12 Rabbit pAb (CAB7786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SUZ12 antibody (CAB7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (CAB7786) at a dilution of 1:500.
