The VRK1 Polyclonal Antibody (CAB19938) is a powerful tool for researchers studying the VRK1 protein, which plays a critical role in cellular processes such as cell cycle regulation and DNA damage response. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.VRK1 is a serine/threonine kinase that is involved in various cellular processes, including cell proliferation, apoptosis, and DNA repair.
Dysregulation of VRK1 has been implicated in diseases such as cancer and neurological disorders, making it a promising target for therapeutic intervention. By using the VRK1 Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of VRK1 in different cell types, aiding in the development of novel therapies for diseases associated with VRK1 dysfunction.
Product Name:
[KO Validated] VRK1 Rabbit Polyclonal Antibody
SKU:
CAB19938
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1).
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Purification Method:
Affinity purification
Gene ID:
7443
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit pAb (CAB19938) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
