The KRR1 Antibody (CAB4487) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity. Predicted to be involved in rRNA processing. Located in chromosome; intercellular bridge; and nuclear lumen.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KRR1 Antibody
SKU:
CAB4487
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HRB2, RIP-1, KRR1
Positive Sample:
HeLa, 293T, A-431, HepG2, Mouse thymus
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
44kDa
Observed MW:
44kDa
Enables RNA binding activity. Predicted to be involved in rRNA processing. Located in chromosome; intercellular bridge; and nuclear lumen.
Purification Method
Affinity purification
Gene ID
11103
RRID
AB_2765708
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KRR1 Rabbit pAb (CAB4487) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal immunofluorescence analysis of U2OS cells using KRR1 Rabbit pAb (CAB4487) at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (CAB4487) at dilution of 1:100 (40x lens)(red). β-Actin (AC004: ACTB Mouse mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).
