The KYNU Monoclonal Antibody (CAB23962) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from mouse hybridoma cells, is highly specific to kynurenine and has been validated for use in various applications, including immunohistochemistry and flow cytometry.Kynurenine is a metabolite produced during the breakdown of tryptophan and has been implicated in a variety of biological processes, including immune tolerance and the regulation of inflammation. By targeting kynurenine, researchers can gain insight into the role of this metabolite in diseases such as cancer, neurodegenerative disorders, and autoimmune diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KYNU Monoclonal Antibody
SKU:
CAB23962
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC62786
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KYNU, KYNUU, kynureninase
Positive Sample:
HeLa, A-549, Mouse liver, Mouse kidney, Rat liver
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
34kDa/52kDa
Observed MW:
52kDa
Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8942
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using KYNU Rabbit mAb (CAB23962) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates, using KYNU Rabbit mAb (CAB23962) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
