The LEF1 Monoclonal Antibody (CAB4473) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been validated for use in immunohistochemistry, immunofluorescence, and other application methods. It targets the LEF1 protein, allowing for precise detection and analysis in a variety of cell types and tissues.LEF1 is a crucial factor in developmental processes, cell differentiation, and tissue homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
LEF1 Monoclonal Antibody
SKU:
CAB4473
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1019
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LEF-1, TCF10, TCF7L3, TCF1ALPHA, LEF1
Positive Sample:
Jurkat, Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
25-58kDa
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51176
RRID
AB_2863281
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LEF1 Rabbit mAb (CAB4473) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
