The Lrwd1 Polyclonal Antibody (CAB21399) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for detecting LRWD1 in human samples, making it ideal for Western blot applications. By targeting the LRWD1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, facilitating research in fields such as molecular biology and cancer biology.LRWD1, also known as Leucine-rich repeat and WD repeat-containing protein 1, is known to play a crucial role in chromatin organization and gene expression regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Lrwd1 Polyclonal Antibody
SKU:
CAB21399
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ORCA, CENP-33, Lrwd1
Cellular Localization:
Chromosome, Cytoplasm, Nucleus, Centromere, Centrosome, Cytoskeleton, Microtubule Organizing Center, Telomere.
Calculated MW:
71kDa
Observed MW:
Refertofigures
The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
222229
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Lrwd1 Rabbit pAb (CAB21399) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
