The Human LXA4 (Lipoxin A4) ELISA Kit is designed for the precise measurement of LXA4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.LXA4 is a lipid mediator known for its anti-inflammatory properties and its role in regulating immune responses. It is involved in resolving inflammation and promoting tissue repair, making it a valuable biomarker for studying inflammatory diseases, autoimmune disorders, and other inflammatory conditions.
By utilizing the Human LXA4 ELISA Kit, researchers can gain insights into the role of LXA4 in various physiological and pathological processes, ultimately advancing our understanding of inflammation and paving the way for potential therapeutic interventions.
Product Name:
LXA4 (Lipoxin A4) ELISA Kit
SKU:
UNES00034
Target:
LXA4 (Lipoxin A4) (UNES00034)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LXA4. During the reaction, LXA4 in the sample or standard competes with a fixed amount of LXA4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LXA4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LXA4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
97-110
89-102
Average (%)
99
104
95
1:4
Range (%)
86-97
89-100
100-115
Average (%)
92
95
105
1:8
Range (%)
89-103
88-100
101-115
Average (%)
95
94
107
1:16
Range (%)
88-101
92-110
95-110
Average (%)
96
100
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
100
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.7
5.1
20.3
2.7
4.9
20.4
Standard deviation
0.1
0.2
1.0
0.1
0.2
0.8
C V (%)
3.7
3.92
4.93
3.7
4.08
3.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of LXA4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes LXA4 in samples. No significant cross-reactivity or interference between LXA4 and analogues was observed.
