The Ly-6C Polyclonal Antibody (CAB23885) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable acetylcholine receptor binding activity and acetylcholine receptor inhibitor activity. Predicted to be involved in acetylcholine receptor signaling pathway. Located in external side of plasma membrane. Is expressed in embryo mesenchyme; gut; liver; metanephros; and nasal cavity. Orthologous to human LY6H (lymphocyte antigen 6 family member H).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Ly-6C Polyclonal Antibody
SKU:
CAB23885
Size:
100μL, 20μL
Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Ly6c, Ly-6C, Ly-6C1
Positive Sample:
Mouse lung, Mouse liver (Low expression control)
Calculated MW:
14KDa
Observed MW:
15kDa
Predicted to enable acetylcholine receptor binding activity and acetylcholine receptor inhibitor activity. Predicted to be involved in acetylcholine receptor signaling pathway. Located in external side of plasma membrane. Is expressed in embryo mesenchyme; gut; liver; metanephros; and nasal cavity. Orthologous to human LY6H (lymphocyte antigen 6 family member H).
Purification Method
Affinity purification
Gene ID
17067
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Ly-6C Rabbit pAb (CAB23885) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.