Magnetic Beads-conjugated anti-DDDDK tag VHH Single Domain antibody (CABE126)
Based on the information provided in the URL, I would write a product description for the Magnetic Beads Conjugated Anti-DDDDK Tag VHH Single Domain Antibody (CABE126) as follows:The Magnetic Beads Conjugated Anti-DDDDK Tag VHH Single Domain Antibody (CABE126) is a powerful tool for researchers studying proteins tagged with the DDDDK epitope. The antibody, derived from llama VHH single domain antibodies, is conjugated to magnetic beads for easy and efficient protein purification and isolation. With high specificity and affinity for the DDDDK tag, this antibody allows for precise and reliable detection and analysis of tagged proteins in various experimental settings.The DDDDK tag, commonly used in protein research for recombinant protein expression and purification, enables easy identification and purification of tagged proteins.
The Magnetic Beads Conjugated Anti-DDDDK Tag VHH Single Domain Antibody (CABE126) simplifies this process by providing a robust and reliable tool for isolating and studying DDDDK-tagged proteins with high efficiency and specificity.Researchers in fields such as molecular biology, biochemistry, and biotechnology can benefit from the convenience and versatility of this antibody for their protein research needs. Whether studying protein interactions, protein localization, or protein expression levels, the Magnetic Beads Conjugated Anti-DDDDK Tag VHH Single Domain Antibody (CABE126) offers a reliable solution for simplifying and improving protein purification
Product Name:
Magnetic Beads-conjugated anti-DDDDK tag VHH Single Domain antibody
SKU:
CABE126
Size:
100uL
Isotype:
VHH
Host Species:
Alpaca
Immunogen:
A synthetic peptide corresponding to DDDDK tag.
Tested Applications:
IP
Recommended Dilution:
IP,30μl-50μl Magnetic Beads for 100μg-300μg extracts of whole cells
Synonyms:
DDDDK;DDDDK tag;DDDDK-tag
Positive Sample:
293T-GSK3B-Flag
Conjugate:
Magnetic Beads
Observed MW:
35kDa/48kDa
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Purification Method:
Affinity purification
Storage Buffer:
Store at 4℃. Avoid freeze / thaw cycles.Buffer: 0.03% sodium azide,20% ethanol
Immunoprecipitation of GSK3B-Flag in 200 µg extracts from 293T cells transfected with GSK3B-Flag using 40 µl Anti-DDDDK (Nanobody) Magnetic Beads mAb (AE126). Western blot analysis was performed using HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095) at 1:5000 dilution.
