The MCM5 Monoclonal Antibody (CAB5008) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, is highly specific for human samples and is validated for use in various experimental applications, including Western blotting and immunofluorescence.MCM5 is a key component of the minichromosome maintenance (MCM) complex, essential for the initiation and elongation of DNA replication. Dysregulation of MCM5 expression has been linked to various diseases, including cancer, making it a valuable target for investigation in oncology research.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCM5 Monoclonal Antibody
SKU:
CAB5008
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1239
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC46, MGORS8, P1-CDC46, MCM5
Positive Sample:
HeLa, MCF7, Mouse spleen, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
82kDa
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Purification Method
Affinity purification
Gene ID
4174
RRID
AB_2863412
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MCM5 Rabbit mAb (CAB5008) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using MCM5 Rabbit mAb (CAB5008) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg MCM5 antibody (CAB5008). Western blot was performed from the immunoprecipitate using MCM5 antibody (CAB5008) at a dilution of 1:1000.
