The MCM5 Monoclonal Antibody (CAB5008) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. RRID AB_2863412 Gene ID 4174 Swiss Prot Synonym CDC46; MGORS8; P1-CDC46; MCM5
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCM5 Monoclonal Antibody
SKU:
CAB5008
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1239
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC46, MGORS8, P1-CDC46, MCM5
Positive Sample:
HeLa, MCF7, Mouse spleen, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
82kDa
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. RRID AB_2863412 Gene ID 4174 Swiss Prot Synonym CDC46; MGORS8; P1-CDC46; MCM5
Purification Method:
Affinity purification
Gene ID:
4174
RRID:
AB_2863412
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MCM5 Rabbit mAb (CAB5008) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using MCM5 Rabbit mAb (CAB5008) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg MCM5 antibody (CAB5008). Western blot was performed from the immunoprecipitate using MCM5 antibody (CAB5008) at a dilution of 1:1000.
