The MDA (Malondialdehyde) ELISA kit offered by Assay Genie is designed for the accurate detection of malondialdehyde levels in various biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a range of research applications.Malondialdehyde is a marker for oxidative stress and lipid peroxidation, which are implicated in a variety of pathological conditions including cardiovascular diseases, neurodegenerative disorders, and inflammatory diseases.
The measurement of malondialdehyde levels can provide valuable insights into the extent of oxidative damage and serve as a biomarker for monitoring disease progression and evaluating potential therapeutic interventions.With its advanced technology and reliable performance, the MDA ELISA kit from Assay Genie is an indispensable tool for researchers studying oxidative stress-related diseases and developing novel treatments to combat these conditions. Order your kit today and unlock new possibilities in your research endeavors.
Product Name:
MDA (Malondialdehyde) ELISA Kit
SKU:
UNES00040
Target:
MDA (Malondialdehyde) (UNES00040)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MDA. During the reaction, MDA in the sample or standard competes with a fixed amount of MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
84-99
89-101
Average (%)
99
91
95
1:4
Range (%)
82-95
88-103
100-113
Average (%)
89
95
105
1:8
Range (%)
86-97
87-102
99-111
Average (%)
92
94
105
1:16
Range (%)
85-96
87-99
94-110
Average (%)
91
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.6
308.7
848.8
111.5
321.0
892.5
Standard deviation
6.1
17.3
37.3
7.0
17.0
46.4
C V (%)
5.57
5.6
4.39
6.28
5.3
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MDA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MDA in samples. No significant cross-reactivity or interference between MDA and analogues was observed.
