The MED16 Polyclonal Antibody (PAC017237) is a powerful tool for researchers studying the MED16 protein, a key component of the Mediator complex involved in gene regulation. This antibody, developed in rabbits, exhibits high specificity and sensitivity in detecting MED16 in human samples, making it ideal for a variety of research applications including Western blotting.MED16 is a critical mediator of transcriptional regulation, playing a pivotal role in controlling gene expression and cellular functions. Its involvement in various biological processes makes it a valuable target for investigation in fields such as molecular biology and cancer research.
By targeting MED16 with this antibody, researchers can gain valuable insights into its function and potential therapeutic applications in disease.The MED16 Polyclonal Antibody is a reliable tool for scientists seeking to understand the role of MED16 in gene regulation and its impact on cellular processes. With its high reactivity and specificity, this antibody is essential for studies aiming to unravel the complexities of gene expression and identify novel therapeutic targets for a range of diseases.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17237(MED16 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hepg2 and hela cell, Primary antibody: PACO17237(MED16 Antibody) at dilution 1/400 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17237(MED16 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Synonyms:
mediator complex subunit 16
UniProt Protein Function:
MED16: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 16 family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Receptor, misc.Chromosomal Location of Human Ortholog: 19p13.3Cellular Component: membrane; nucleoplasm; Srb-mediator complexMolecular Function: protein binding; receptor activity; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription cofactor activityBiological Process: androgen receptor signaling pathway; positive regulation of transcription, DNA-dependent; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
