The MED4 Antibody (CAB9150) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets human samples and is validated for use in Western blot applications. It effectively binds to the MED4 protein, allowing for accurate detection and analysis in a variety of cell types.MED4 is known for its role in regulating important cellular functions, making it a key target in research related to cell biology and molecular mechanisms. By studying the activity and expression of MED4, researchers can gain valuable insights into processes such as cell growth, division, and signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
MED4 Antibody
SKU:
CAB9150
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARC36, VDRIP, DRIP36, TRAP36, HSPC126, MED4
Positive Sample:
Jurkat, SH-SY5Y, HL-60, SW480, HT-1080
Cellular Localization:
Nucleus.
Calculated MW:
30kDa
Observed MW:
36kDa
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
29079
RRID
AB_2770331
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MED4 Rabbit pAb (CAB9150) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
