The MKL1 Antibody (CAB12598) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody has high reactivity with human samples and has been validated for use in Western blot applications.MKL1, also known as Megakaryoblastic Leukemia 1, plays a crucial role in regulating gene expression in response to various signaling pathways, including those involved in cell growth and differentiation. Dysregulation of MKL1 has been implicated in diseases such as cancer and cardiovascular disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MKL1 Antibody
SKU:
CAB12598
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAL, MKL, BSAC, MKL1, MRTF-A
Positive Sample:
TE-1, HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
99kDa
Observed MW:
145kDa
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
57591
RRID
AB_2759440
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MKL1 Rabbit pAb (CAB12598) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
