The MKRN1 Polyclonal Antibody (PAC056202) is a valuable tool for researchers studying MKRN1, a protein involved in cell regulation and cancer development. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MKRN1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in cancer biology and cell signaling pathways.MKRN1, also known as makorin ring finger protein 1, is a key player in the regulation of gene expression and protein degradation, making it a promising target for cancer therapy research. Its involvement in various cellular processes underscores its potential as a biomarker for cancer diagnosis and treatment.
By elucidating the function of MKRN1, researchers can gain insights into the mechanisms driving tumorigenesis and potentially identify novel therapeutic strategies for cancer patients.Overall, the MKRN1 Polyclonal Antibody is a valuable resource for scientists investigating the role of MKRN1 in cancer biology and beyond. Its high specificity and sensitivity make it a reliable tool for exploring the functional significance of MKRN1 in various biological contexts, paving the way for innovative discoveries in cancer research and therapeutic development.
Antibody Name:
MKRN1 Antibody (PACO56202)
Antibody SKU:
PACO56202
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human E3 ubiquitin-protein ligase makorin-1 protein (109-209AA)
IHC image of PACO56202 diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]
