The Monkey EPO (Erythropoietin) ELISA Kit is designed to accurately measure erythropoietin levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it an excellent choice for various research applications. Erythropoietin is a key hormone involved in the regulation of red blood cell production, making it essential for maintaining oxygen levels in the body.
Dysregulation of erythropoietin levels can lead to conditions such as anemia and kidney disease, highlighting its importance as a biomarker in studying these diseases and potential therapeutic interventions. Whether you are studying the effects of hypoxia, kidney function, or erythropoiesis in monkeys, the Monkey EPO ELISA Kit offers a reliable and efficient solution for accurately quantifying erythropoietin levels in biological samples.
Product Name:
Monkey EPO (Erythropoietin) ELISA Kit
SKU:
MKES00020
Target:
Monkey EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey EPO. You can calculate the concentration of Monkey EPO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
93-106
97-112
Average (%)
91
100
105
1:4
Range (%)
86-101
81-95
87-101
Average (%)
93
87
92
1:8
Range (%)
88-103
83-95
89-100
Average (%)
95
88
94
1:16
Range (%)
92-104
81-93
88-100
Average (%)
97
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.6
256.09
923.49
108.32
268.79
923.49
Standard deviation
6.45
14.57
46.45
7.13
13.76
43.4
C V (%)
6.54
5.69
5.03
6.58
5.12
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey EPO in samples. No significant cross-reactivity or interference between Monkey EPO and analogues was observed.
