Description
Monkey FG (Fibrinogen) ELISA Kit
The Monkey FG (Fibrinogen) ELISA Kit is a specialized assay designed to quantitatively measure fibrinogen levels in monkey biological samples. Fibrinogen is a crucial protein involved in the blood coagulation process, playing a key role in forming blood clots and maintaining hemostasis.
This ELISA kit enables researchers to accurately assess fibrinogen concentrations in monkey samples, providing insights into coagulation pathways and clot formation. Monitoring fibrinogen levels is essential for understanding blood clotting mechanisms, diagnosing clotting disorders, and studying conditions related to hemostasis and thrombosis. With exceptional sensitivity and specificity, the Monkey FG ELISA Kit ensures precise and reproducible results, allowing researchers to investigate coagulation processes in primate models. Manufactured under stringent quality control standards, this kit offers reliable performance and user-friendly protocols, making it an excellent tool for research focused on hemostasis and coagulation in monkeys.
| Product Name: | Monkey FG (Fibrinogen) ELISA Kit |
| Product Code: | AEES00714 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 4.5h |
| Reactivity: | Monkey |
| Detection Range: | 9.38-600 ng/mL |
| Sensitivity: | 5.63 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Monkey FG in samples. No significant cross-reactivity or interference between Monkey FG and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Monkey FG concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey FG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey FG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey FG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey FG. You can calculate the concentration of Monkey FG in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey FG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey FG were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 31.04 | 68.51 | 294.20 | 33.16 | 67.05 | 299.55 |
| Standard deviation | 1.99 | 3.43 | 12.39 | 2.30 | 3.89 | 11.86 |
| CV (%) | 6.41 | 5.01 | 4.21 | 6.94 | 5.80 | 3.96 |
Recovery
The recovery of Monkey FG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 98 |
| EDTA plasma (n=8) | 93-104 | 99 |
| Cell culture media (n=8) | 85-95 | 90 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
