The Monkey Immunoglobulin E (IgE) ELISA Kit is a specialized assay designed for the precise quantification of IgE levels in samples from monkey species. Immunoglobulin E (IgE) is an essential antibody class involved in allergic responses and parasitic defense mechanisms in primates, playing a crucial role in the immune system's hypersensitivity reactions. This ELISA kit allows researchers to accurately measure IgE concentrations in monkey samples, providing valuable insights into allergic reactions, immune responses to parasites, and the pathophysiology of hypersensitivity disorders. By quantifying IgE levels, researchers can enhance their understanding of immune-mediated conditions, assess allergic sensitization, and monitor the efficacy of interventions targeting IgE-mediated pathways.
With exceptional sensitivity and specificity, the Monkey IgE ELISA Kit ensures accurate and reproducible results, enabling researchers to conduct in-depth investigations into the role of IgE in primate immunity with confidence. Manufactured under strict quality control guidelines, this kit delivers robust performance and user-friendly operation, making it a reliable tool for immunological research and allergy-related studies in monkey models.
Product Name:
Monkey IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00681
Target:
Monkey IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey IgE. You can calculate the concentration of Monkey IgE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
91-106
87-99
Average (%)
101
98
92
1:4
Range (%)
95-110
90-102
86-101
Average (%)
103
97
93
1:8
Range (%)
97-112
91-101
86-97
Average (%)
103
96
91
1:16
Range (%)
97-110
93-104
86-96
Average (%)
102
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
92-106
100
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
218.42
623.82
1549.76
208.65
586.76
1546.69
Standard deviation
11.77
35.31
80.28
13.54
35.09
65.89
C V (%)
5.39
5.66
5.18
6.49
5.98
4.26
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey IgE concentrations in .
Specificity:
This kit recognizes Monkey IgE in samples. No significant cross-reactivity or interference between Monkey IgE and analogues was observed.
