The Monkey INS (Insulin) ELISA Kit is a specialized assay designed for the quantitative detection of insulin levels in various biological samples from monkeys. Insulin is a pivotal hormone involved in regulating glucose metabolism, energy storage, and homeostasis in primates. This ELISA kit provides researchers with a precise tool to measure insulin concentrations, facilitating studies on metabolic processes, diabetes, and endocrine function in primate models. Accurate measurement of insulin is crucial for understanding its role in glucose regulation, energy metabolism, and metabolic disorders.
The Monkey INS ELISA Kit ensures outstanding sensitivity and specificity, enabling reliable and reproducible results tailored to your research needs. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for investigative studies focusing on insulin physiology and related pathologies.
Product Name:
Monkey INS (Insulin) ELISA Kit
SKU:
AEES00270
Target:
Monkey INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 nIU/mL
Detection range:
15.63-1000 nIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey INS. You can calculate the concentration of Monkey INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
83-98
88-97
Average (%)
105
90
92
1:4
Range (%)
87-97
93-105
87-100
Average (%)
92
98
92
1:8
Range (%)
88-95
87-98
87-100
Average (%)
92
93
94
1:16
Range (%)
85-94
100-107
96-103
Average (%)
91
104
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-102
96
EDTA plasma (n=5)
82-93
90
Cell culture media (n=5)
83-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.23
9.5
0.89
2.07
9.42
Standard deviation
0.05
0.11
0.45
0.05
0.1
0.5
C V (%)
5.38
4.93
4.74
5.62
4.83
5.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey INS in samples. No significant cross-reactivity or interference between Monkey INS and analogues was observed.
