The Monkey TSH (Thyroid Stimulating Hormone) Elisa Kit is a reliable tool for accurately measuring levels of TSH in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides precise and reproducible results, making it well-suited for a variety of research applications.TSH, also known as thyrotropin, plays a crucial role in regulating thyroid function by stimulating the production and release of thyroid hormones.
Imbalances in TSH levels can indicate thyroid dysfunction, making it an important biomarker for studying thyroid conditions and developing therapeutic interventions.Overall, the Monkey TSH Elisa Kit offers researchers a valuable tool for investigating the role of TSH in monkey physiology and pathology, providing insights into thyroid function and potential treatment strategies for thyroid disorders.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey TSH. You can calculate the concentration of Monkey TSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
97-107
98-113
Average (%)
104
102
104
1:4
Range (%)
89-102
82-94
87-103
Average (%)
94
87
94
1:8
Range (%)
90-103
80-92
88-100
Average (%)
97
86
95
1:16
Range (%)
85-99
81-95
86-97
Average (%)
92
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.68
6.13
20.25
2.82
5.92
21.71
Standard deviation
0.17
0.37
0.85
0.19
0.32
0.71
C V (%)
6.34
6.04
4.2
6.74
5.41
3.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey TSH in samples. No significant cross-reactivity or interference between Monkey TSH and analogues was observed.
