The MonoMethyl-Histone H3-R2 Monoclonal Antibody (CAB19645) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and sensitive for detecting the monomethyl histone H3R2 modification in various cell types and tissues.Histone modifications, including methylation at specific residues, play a crucial role in regulating gene expression and chromatin structure. The monomethylation of histone H3R2 is involved in transcriptional regulation and has been linked to various cellular processes, including development, differentiation, and disease.
This antibody is validated for use in WB, ELISA, DB, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H3-R2 Monoclonal Antibody
SKU:
CAB19645
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:
ARC0124
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2862712
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-R2 Rabbit mAb (CAB19645) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): H3 protein. Exposure time: 60 s.
Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-R2 antibody (CAB19645) at 1:1000 dilution.
