The MonoMethyl-YAP1-K342 Antibody (CAB18651) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the monomethylated form of the YAP1 protein at lysine 342, enabling precise detection and analysis in various cell types.YAP1, a transcriptional coactivator, plays a crucial role in controlling gene expression pathways involved in cell proliferation, survival, and tissue regeneration. Dysregulation of YAP1 is associated with various diseases, including cancer, making it an attractive target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MonoMethyl-YAP1-K342 Antibody
SKU:
CAB18651
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SPKC Q
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
10413
RRID
AB_2862389
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-YAP1-K342 Rabbit pAb (CAB18651) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
