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MonoMethyl-H2B-E105 Rabbit Polyclonal Antibody (CAB20230)

SKU:
CAB20230
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
H2B.1
H2B/f
H2BFF
HIST1H2BB
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MonoMethyl-H2B-E105 Rabbit Polyclonal Antibody
SKU:CAB20230
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic monomethylated peptide around E105 of human H2B (NP_066406.1).
Sequence:LPGE L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:H2B.1; H2B/f; H2BFF; HIST1H2BB; MonoMethyl-H2B-E105
Positive Sample:HeLa,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:cytosol, nucleoplasm, nucleus
Calculated MW:14kDa
Observed MW:15kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:3018
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using MonoMethyl-H2B-E105 Rabbit pAb (CAB20230) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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