The Mouse Activin A CLIA Kit is specifically designed for the quantitative measurement of Activin A levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for various applications.Activin A is a member of the TGF-beta superfamily and plays a critical role in regulating cell growth, differentiation, and development. It is involved in various physiological processes, including embryogenesis, immune response, and the regulation of reproductive functions.
In addition, Activin A has been implicated in several disease states, such as cancer, inflammation, and fibrosis, making it a valuable tool for studying disease mechanisms and potential therapeutic interventions.Overall, the Mouse Activin A CLIA Kit is an essential tool for researchers studying the role of Activin A in various biological processes and disease conditions, offering accurate and reliable quantitative data for experimental analysis.
Product Name:
Mouse ACV-A (Activin A) CLIA Kit
SKU:
MOES00001
Target:
Mouse ACV-A (Activin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACV-A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ACV-A. You can calculate the concentration of Mouse ACV-A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-112
103-119
Average (%)
96
106
109
1:4
Range (%)
97-108
93-104
97-112
Average (%)
103
98
104
1:8
Range (%)
89-103
90-105
99-114
Average (%)
96
96
106
1:16
Range (%)
86-97
94-108
89-104
Average (%)
92
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.95
138.75
399.01
51.55
137.29
426.41
Standard deviation
4.43
16.26
27.13
5.26
15.94
29.76
C V (%)
9.05
11.72
6.8
10.2
11.61
6.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ACV-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ACV-A in samples. No significant cross-reactivity or interference between Mouse ACV-A and analogues was observed.
