The Mouse AGRP (Agouti-Related Protein) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of AGRP levels in mouse serum, plasma, and cell culture supernatants. This kit is essential for researchers studying the role of AGRP in regulating energy balance, food intake, and metabolism.AGRP is a vital neuropeptide involved in the regulation of appetite and energy expenditure, making it a key player in conditions such as obesity and metabolic disorders.
By accurately measuring AGRP levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop targeted therapies.With its reliable and reproducible results, the Mouse AGRP CLIA Kit is an invaluable tool for investigating the role of AGRP in physiological and pathological processes, offering researchers the opportunity to advance their studies and make significant contributions to the field of metabolism and energy homeostasis.
Product Name:
Mouse AgRP (Agouti Related Protein) CLIA Kit
SKU:
MOES00615
Target:
Mouse AgRP (Agouti Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AgRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AgRP. You can calculate the concentration of Mouse AgRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
98-116
102-117
Average (%)
100
106
108
1:4
Range (%)
97-112
91-104
103-118
Average (%)
104
96
110
1:8
Range (%)
89-102
99-116
93-107
Average (%)
95
107
99
1:16
Range (%)
102-116
87-98
95-108
Average (%)
108
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
103
EDTA plasma (n=5)
101-117
108
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.83
43.64
211.32
23.13
44.35
199.9
Standard deviation
2.79
3.47
24.22
2.1
4.45
13.23
C V (%)
11.24
7.95
11.46
9.08
10.03
6.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AgRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AgRP in samples. No significant cross-reactivity or interference between Mouse AgRP and analogues was observed.
