The Mouse AGT (Angiotensinogen) CLIA Kit is specifically designed for the precise measurement of angiotensinogen levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research applications.Angiotensinogen is a key protein in the renin-angiotensin system, playing a pivotal role in regulating blood pressure and fluid balance. Dysregulation of angiotensinogen is associated with conditions such as hypertension, kidney disorders, and cardiovascular diseases, making it a vital biomarker for understanding these diseases and developing potential treatments.
With the Mouse AGT CLIA Kit, researchers can accurately assess angiotensinogen levels in mouse samples, aiding in the advancement of scientific knowledge and the development of innovative therapies for related medical conditions.
Product Name:
Mouse AGT (Angiotensinogen) CLIA Kit
SKU:
MOES00073
Target:
Mouse AGT (Angiotensinogen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AGT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AGT. You can calculate the concentration of Mouse AGT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
100-113
96-108
Average (%)
100
107
102
1:4
Range (%)
101-112
96-110
104-121
Average (%)
107
103
110
1:8
Range (%)
101-115
93-105
88-101
Average (%)
107
99
96
1:16
Range (%)
97-113
98-112
96-112
Average (%)
103
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
386.35
1214.92
3682.52
416.9
1261.18
3471.51
Standard deviation
42.19
125.62
257.78
35.94
105.31
289.52
C V (%)
10.92
10.34
7.0
8.62
8.35
8.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AGT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AGT in samples. No significant cross-reactivity or interference between Mouse AGT and analogues was observed.
