The Mouse Angiotensin I (ANGI) ELISA Kit offered by Assay Genie is a reliable and accurate tool for detecting levels of angiotensin I in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reproducible results for a variety of research applications.Angiotensin I is a key component in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. Dysregulation of this system has been implicated in various cardiovascular and renal diseases, making angiotensin I an important biomarker for studying these conditions and developing potential therapies.
The Mouse Angiotensin I (ANGI) ELISA Kit from Assay Genie provides researchers with a user-friendly and reliable tool for accurately measuring angiotensin I levels in mouse samples, facilitating valuable insights into the role of this peptide in physiological and pathological processes.
Product Name:
Mouse Angiotensin I (AngI) ELISA Kit
Product Code:
MODL00068
Size:
96 Assays
Target:
AngI
Synonyms:
Ang-I, Angiotensin-1
Detection Method:
Sandwich
Range:
4.69-300 pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
