Mouse ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit
The Mouse ANGPTL2 (Angiopoietin-like protein 2) CLIA Kit is a powerful tool for the quantitative measurement of ANGPTL2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.ANGPTL2 is an important protein involved in inflammation, metabolism, and cardiovascular diseases. It plays a critical role in regulating lipid metabolism, insulin sensitivity, and inflammation, making it a valuable biomarker for studying these processes and developing potential therapeutic interventions.
The Mouse ANGPTL2 CLIA Kit from AssayGenie is a valuable tool for researchers looking to study the role of ANGPTL2 in various physiological and pathological conditions in mice. With its high sensitivity and specificity, this kit provides the precision and accuracy needed for robust and reproducible results in research studies.
Product Name:
Mouse ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit
SKU:
MOES00071
Target:
Mouse ANGPTL2 (Angiopoietin Like Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANGPTL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANGPTL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANGPTL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANGPTL2. You can calculate the concentration of Mouse ANGPTL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
91-105
91-103
Average (%)
103
98
97
1:4
Range (%)
84-98
93-107
96-111
Average (%)
91
99
102
1:8
Range (%)
86-97
93-109
93-109
Average (%)
92
101
100
1:16
Range (%)
101-115
102-119
101-114
Average (%)
106
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.27
118.45
319.94
46.0
109.47
322.58
Standard deviation
5.43
12.63
32.09
5.26
8.53
31.77
C V (%)
12.85
10.66
10.03
11.43
7.79
9.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANGPTL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANGPTL2 in samples. No significant cross-reactivity or interference between Mouse ANGPTL2 and analogues was observed.
