Mouse ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit
The Mouse ANGPTL4 (Angiopoietin-Like Protein 4) CLIA Kit is a powerful tool for detecting levels of ANGPTL4 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.ANGPTL4 is a key player in metabolic regulation, lipid metabolism, and inflammation, making it a valuable biomarker for studying obesity, diabetes, and cardiovascular diseases.
Understanding the role of ANGPTL4 can provide insight into the mechanisms underlying these conditions and aid in the development of targeted therapies.Overall, the Mouse ANGPTL4 CLIA Kit is an essential resource for researchers looking to investigate the functions and implications of ANGPTL4 in various physiological and pathological processes.
Product Name:
Mouse ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit
SKU:
MOES00072
Target:
Mouse ANGPTL4 (Angiopoietin Like Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANGPTL-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANGPTL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANGPTL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANGPTL-4. You can calculate the concentration of Mouse ANGPTL-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
103-116
85-98
Average (%)
102
109
92
1:4
Range (%)
90-102
87-99
99-117
Average (%)
97
92
107
1:8
Range (%)
89-104
96-111
95-107
Average (%)
95
103
101
1:16
Range (%)
98-111
97-111
94-108
Average (%)
105
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
108
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.15
188.49
908.13
108.67
201.16
891.14
Standard deviation
11.22
18.06
101.44
10.87
14.95
81.9
C V (%)
11.09
9.58
11.17
10.0
7.43
9.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANGPTL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANGPTL-4 in samples. No significant cross-reactivity or interference between Mouse ANGPTL-4 and analogues was observed.
