The Mouse ANP (Atrial Natriuretic Peptide) ELISA Kit is specifically designed for the accurate quantification of ANP levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers in various fields.ANP is a key peptide involved in regulating blood pressure and fluid balance in the body. It plays a critical role in cardiovascular health, making it a valuable biomarker for studying heart-related conditions and potential therapeutic interventions.
With this ELISA kit, researchers can efficiently measure ANP levels and advance their understanding of cardiovascular physiology and pathology in mouse models.Overall, the Mouse ANP ELISA Kit from Assay Genie provides a reliable tool for researchers to conduct accurate and comprehensive studies on ANP biology and its implications in cardiovascular health.
Product Name:
Mouse ANP (Atrial Natriuretic Peptide) ELISA Kit
SKU:
MOES00752
Target:
Mouse ANP (Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
97-112
90-104
Average (%)
106
103
97
1:4
Range (%)
90-101
89-101
99-110
Average (%)
96
95
104
1:8
Range (%)
89-102
87-98
94-109
Average (%)
94
93
101
1:16
Range (%)
86-100
92-108
96-111
Average (%)
93
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.5
157.2
342.3
43.0
163.1
362.8
Standard deviation
3
7.7
13.3
2.6
7.2
12.7
C V (%)
6.32
4.9
3.89
6.05
4.41
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed.
