The Mouse Apo-C1 (Apolipoprotein C1) CLIA Kit is specifically designed for the quantitative detection of mouse Apo-C1 levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Apo-C1 is a key protein involved in lipid metabolism and lipoprotein regulation. It plays a critical role in lipid transport and metabolism, making it a valuable biomarker for studying conditions such as cardiovascular disease, hyperlipidemia, and metabolic disorders.
With its high-quality components and user-friendly protocol, the Mouse Apo-C1 CLIA Kit is an essential tool for researchers looking to investigate Apo-C1 levels and understand its role in various physiological and pathological processes.
Product Name:
Mouse APO-C1 (Apolipoprotein C1) CLIA Kit
SKU:
MOES00085
Target:
Mouse APO-C1 (Apolipoprotein C1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 ng/mL
Detection range:
12.50-800 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse APO-C1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse APO-C1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse APO-C1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse APO-C1. You can calculate the concentration of Mouse APO-C1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
95-111
86-97
Average (%)
105
102
92
1:4
Range (%)
96-112
87-101
99-114
Average (%)
104
93
106
1:8
Range (%)
99-116
101-116
91-103
Average (%)
106
107
96
1:16
Range (%)
84-98
88-103
101-115
Average (%)
91
95
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.04
101.01
366.5
41.87
91.02
362.49
Standard deviation
4.63
9.03
35.7
3.36
8.34
26.82
C V (%)
10.51
8.94
9.74
8.02
9.16
7.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse APO-C1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse APO-C1 in samples. No significant cross-reactivity or interference between Mouse APO-C1 and analogues was observed.
