The Mouse AQP-4 (Aquaporin 4) CLIA Kit is specifically designed for the quantitative detection of AQP-4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.AQP-4 is a key protein involved in water homeostasis and cell volume regulation, particularly in the central nervous system. Dysregulation of AQP-4 has been implicated in various neurological disorders such as brain edema, multiple sclerosis, and neuromyelitis optica, highlighting its importance as a potential therapeutic target.
By utilizing the Mouse AQP-4 CLIA Kit, researchers can gain valuable insights into the role of AQP-4 in physiological and pathological processes, paving the way for the development of novel treatment strategies for a range of neurological conditions.
Product Name:
Mouse AQP-4 (Aquaporin 4) CLIA Kit
SKU:
MOES00098
Target:
Mouse AQP-4 (Aquaporin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AQP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AQP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AQP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AQP-4. You can calculate the concentration of Mouse AQP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
102-118
98-111
Average (%)
94
109
103
1:4
Range (%)
94-108
90-102
85-101
Average (%)
99
96
92
1:8
Range (%)
87-101
98-111
86-98
Average (%)
94
105
91
1:16
Range (%)
94-108
103-117
83-96
Average (%)
100
109
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
103
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.59
321.37
714.95
103.65
297.27
746.83
Standard deviation
9.16
25.9
43.33
10.31
25.27
61.91
C V (%)
9.11
8.06
6.06
9.95
8.5
8.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AQP-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AQP-4 in samples. No significant cross-reactivity or interference between Mouse AQP-4 and analogues was observed.
