The Mouse AR (Amphiregulin) CLIA Kit is a powerful tool for researchers looking to accurately measure amphiregulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Amphiregulin is a key growth factor that plays a critical role in various biological processes, including tissue regeneration, wound healing, and immune response modulation.
Dysregulation of amphiregulin has been linked to a range of diseases, such as cancer, inflammatory disorders, and autoimmune conditions, making it a valuable biomarker for studying disease mechanisms and developing potential therapeutic interventions.With the Mouse AR (Amphiregulin) CLIA Kit, researchers can confidently explore the role of amphiregulin in health and disease, opening up new possibilities for innovative research and translational medicine.
Product Name:
Mouse AR (Amphiregulin) CLIA Kit
SKU:
MOES00420
Target:
Mouse AR (Amphiregulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AR. You can calculate the concentration of Mouse AR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
89-106
86-99
Average (%)
110
97
93
1:4
Range (%)
94-109
91-105
102-116
Average (%)
102
97
109
1:8
Range (%)
86-99
97-113
97-111
Average (%)
93
105
105
1:16
Range (%)
95-109
89-102
103-116
Average (%)
103
96
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.7
64.64
182.33
25.19
65.3
194.06
Standard deviation
2.21
7.41
16.59
2.75
6.28
17.39
C V (%)
8.6
11.46
9.1
10.92
9.62
8.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AR in samples. No significant cross-reactivity or interference between Mouse AR and analogues was observed.
