The Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit is designed for the accurate and precise measurement of BALP levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for various research applications.BALP is a key enzyme found in bone tissue that plays a critical role in bone mineralization and turnover.
Monitoring BALP levels can provide valuable insights into bone health and metabolism, making it a valuable biomarker for studying bone-related disorders and evaluating potential treatments.Overall, the Mouse BALP CLIA Kit is a valuable tool for researchers seeking to analyze bone alkaline phosphatase levels in mouse models, providing essential data for further understanding bone physiology and pathology.
Product Name:
Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit
SKU:
MOES00127
Target:
Mouse BALP (Bone Alkaline Phosphatase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BALP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BALP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BALP. You can calculate the concentration of Mouse BALP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
88-104
90-105
Average (%)
108
95
96
1:4
Range (%)
97-112
102-118
86-97
Average (%)
105
109
92
1:8
Range (%)
90-104
96-111
92-106
Average (%)
97
104
100
1:16
Range (%)
92-103
94-108
91-104
Average (%)
98
99
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
107
EDTA plasma (n=5)
95-113
103
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.75
287.52
947.47
100.48
288.62
1014.09
Standard deviation
11.38
28.78
65.94
10.53
23.06
66.42
C V (%)
11.76
10.01
6.96
10.48
7.99
6.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BALP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BALP in samples. No significant cross-reactivity or interference between Mouse BALP and analogues was observed.
