The Mouse BDNF (Brain-Derived Neurotrophic Factor) ELISA Kit is specifically designed for the accurate quantification of BDNF levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.BDNF is a key protein in the brain that is involved in promoting the growth, survival, and differentiation of neurons. It plays a critical role in various neurological processes, including learning, memory, and mood regulation.
Dysregulation of BDNF has been implicated in several neurological disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse BDNF ELISA Kit is an essential tool for researchers working in the field of neuroscience and studying the role of BDNF in brain function and neurodegenerative diseases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BDNF. You can calculate the concentration of Mouse BDNF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
86-96
94-109
Average (%)
92
91
101
1:4
Range (%)
98-110
80-92
92-107
Average (%)
103
86
100
1:8
Range (%)
97-110
84-97
97-111
Average (%)
105
91
103
1:16
Range (%)
99-114
82-97
95-109
Average (%)
107
88
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.3
200.7
906.7
117.2
182.0
864.0
Standard deviation
7.5
9.0
29.0
6.6
7.6
35.4
C V (%)
6.93
4.48
3.2
5.63
4.18
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse BDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BDNF in samples. No significant cross-reactivity or interference between Mouse BDNF and analogues was observed.
Konstantinos Mesiakaris et al.
Cannabidiol Modulation of Nicotine-Induced Toxicity: Assessing Effects on Behavior, Brain-Derived Neurotrophic Factor, and Oxidative Stress in C57BL/6 Male Mice
