The Mouse Beta-TG (beta-Thromboglobulin) CLIA Kit is specifically designed for the quantitative detection of beta-thromboglobulin levels in mouse serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and accuracy, ensuring precise and reproducible results for a variety of research applications.Beta-thromboglobulin is a key protein involved in platelet activation and aggregation, playing a critical role in the clotting process and inflammation. Monitoring beta-thromboglobulin levels can provide valuable insights into thrombotic disorders, cardiovascular diseases, and inflammatory conditions, making this kit indispensable for studying these pathologies and potential therapeutic interventions.
With its user-friendly protocol and dependable performance, the Mouse Beta-TG CLIA Kit from Assay Genie is a valuable tool for researchers seeking to explore the intricate mechanisms of platelet function and vascular health in mouse models.
Product Name:
Mouse beta-TG (beta-Thromboglobulin) CLIA Kit
SKU:
MOES00600
Target:
Mouse beta-TG (beta-Thromboglobulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse β-TG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse β-TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse β-TG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse β-TG. You can calculate the concentration of Mouse β-TG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
104-120
94-110
Average (%)
93
109
101
1:4
Range (%)
94-109
98-110
93-109
Average (%)
102
104
100
1:8
Range (%)
87-98
99-116
94-108
Average (%)
92
107
100
1:16
Range (%)
97-110
92-107
102-119
Average (%)
103
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
98-111
105
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.13
24.99
77.85
9.56
23.4
73.15
Standard deviation
0.76
2.38
8.52
1.14
2.24
4.97
C V (%)
8.32
9.52
10.94
11.92
9.57
6.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse β-TG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse β-TG in samples. No significant cross-reactivity or interference between Mouse β-TG and analogues was observed.
