The Mouse bFGF (FGF2) CLIA Kit is specifically designed for the quantitative detection of basic fibroblast growth factor (bFGF or FGF2) levels in mouse samples. This kit offers high sensitivity and accuracy, allowing for precise measurements in serum, plasma, and tissue culture supernatants.bFGF, also known as FGF2, is a critical growth factor involved in various cellular processes, including cell proliferation, differentiation, and tissue repair. It plays a key role in angiogenesis, promoting the formation of new blood vessels and supporting tissue regeneration.
Dysregulation of bFGF levels has been implicated in diseases such as cancer, cardiovascular disorders, and neurological conditions, highlighting its importance as a biomarker for disease progression and therapeutic development.With its reliable performance and versatility, the Mouse bFGF (FGF2) CLIA Kit is an essential tool for researchers studying angiogenesis, cell growth, and tissue regeneration in mouse models, offering valuable insights into the role of bFGF in health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse bFGF/FGF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse bFGF/FGF2. You can calculate the concentration of Mouse bFGF/FGF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
101-117
87-99
Average (%)
106
107
93
1:4
Range (%)
90-105
94-106
90-105
Average (%)
96
101
97
1:8
Range (%)
90-104
98-115
85-98
Average (%)
97
105
90
1:16
Range (%)
94-109
87-101
86-98
Average (%)
102
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.47
67.82
168.19
21.53
64.21
177.44
Standard deviation
2.57
5.99
16.21
2.73
6.54
12.14
C V (%)
10.95
8.83
9.64
12.68
10.19
6.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse bFGF/FGF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse bFGF/FGF2 in samples. No significant cross-reactivity or interference between Mouse bFGF/FGF2 and analogues was observed.
