The Mouse BK (Bradykinin) ELISA Kit (MOES00781) offered by AssayGenie is a cutting-edge research tool designed for the precise measurement of bradykinin levels in mouse serum, plasma, and tissue culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results that are vital for a wide array of experimental applications.Bradykinin is a key signaling molecule in inflammation and pain pathways, playing a crucial role in vasodilation, smooth muscle contraction, and permeability of blood vessels.
Dysregulation of bradykinin levels has been implicated in various pathological conditions, including hypertension, edema, and inflammatory disorders. As such, this ELISA kit is an invaluable resource for researchers studying the role of bradykinin in health and disease, paving the way for potential therapeutic interventions and advancements in the field of biomedical research.Experience unparalleled precision and reliability with the Mouse BK (Bradykinin) ELISA Kit (MOES00781) from AssayGenie - your trusted partner in cutting-edge research solutions.
Product Name:
Mouse BK (Bradykinin) ELISA Kit
SKU:
MOES00781
Target:
Mouse BK (Bradykinin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BK. During the reaction, BK in the sample or standard competes with a fixed amount of BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BK. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BK in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
96-113
98-111
Average (%)
99
103
104
1:4
Range (%)
88-103
88-99
94-106
Average (%)
94
93
100
1:8
Range (%)
88-100
91-105
96-107
Average (%)
95
98
101
1:16
Range (%)
92-104
92-103
98-115
Average (%)
97
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5
15.5
44.1
4.6
15.2
45.5
Standard deviation
0.3
0.7
2.1
0.2
0.8
1.6
C V (%)
6
4.52
4.76
4.35
5.26
3.52
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of BK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes BK in samples. No significant cross-reactivity or interference between BK and analogues was observed.
