The Mouse BNP (Brain Natriuretic Peptide) ELISA Kit is specifically designed for the accurate measurement of BNP levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing dependable and consistent results for a variety of research applications.BNP is a vital peptide hormone that plays a key role in regulating blood pressure and fluid balance. It is predominantly produced and released by the heart in response to increased pressure and volume overload, making it an important biomarker for cardiovascular diseases such as heart failure and hypertension.
With its reliable performance and broad utility, the Mouse BNP ELISA Kit is an essential tool for researchers studying cardiovascular health and developing innovative therapies for related conditions.
Product Name:
Mouse BNP (Brain Natriuretic Peptide) ELISA Kit
SKU:
MOES00783
Target:
Mouse BNP (Brain Natriuretic Peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-95
98-113
95-110
Average (%)
90
103
102
1:4
Range (%)
83-99
92-106
95-112
Average (%)
90
98
103
1:8
Range (%)
87-101
93-106
94-108
Average (%)
92
99
100
1:16
Range (%)
88-103
90-102
97-110
Average (%)
95
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.4
130.4
492.6
52.7
130.3
444.7
Standard deviation
3.2
7.2
22.7
2.7
7.8
17.3
C V (%)
6.23
5.52
4.61
5.12
5.99
3.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
