The Mouse BTC (Beta Cellulin) CLIA Kit is specifically designed for the quantitative measurement of Beta Cellulin levels in mouse serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reproducible results, making it suitable for a variety of research applications.Beta Cellulin is a key growth factor that plays a critical role in regulating cell growth, proliferation, and differentiation. It is involved in various physiological processes such as development, tissue repair, and inflammation.
Dysregulation of Beta Cellulin expression has been linked to various diseases including cancer and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse BTC (Beta Cellulin) CLIA Kit offers researchers a reliable tool for studying the role of Beta Cellulin in biological processes and disease pathogenesis, with the potential for advancing our understanding and treatment of related conditions.
Product Name:
Mouse BTC (Beta Cellulin) CLIA Kit
SKU:
MOES00115
Target:
Mouse BTC (Beta Cellulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BTC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BTC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BTC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BTC. You can calculate the concentration of Mouse BTC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
99-110
94-109
Average (%)
101
104
100
1:4
Range (%)
96-107
95-112
97-112
Average (%)
102
103
105
1:8
Range (%)
95-107
96-112
99-113
Average (%)
102
102
105
1:16
Range (%)
93-109
87-99
89-103
Average (%)
99
93
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.7
23.66
81.32
11.53
25.83
79.6
Standard deviation
1.32
2.63
7.57
1.18
2.18
7.56
C V (%)
12.34
11.12
9.31
10.23
8.44
9.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BTC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BTC in samples. No significant cross-reactivity or interference between Mouse BTC and analogues was observed.
