The Mouse C3 (Complement 3) ELISA Kit is specifically designed for the precise measurement of complement 3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it perfect for various research purposes.Complement 3 is a vital component of the complement system, which plays a crucial role in the body's immune response. It is involved in various processes such as inflammation, opsonization, and cell lysis.
Dysregulation of complement 3 has been linked to several diseases, including autoimmune disorders, infections, and inflammatory conditions.Overall, the Mouse C3 ELISA Kit is a valuable tool for researchers studying the role of complement 3 in health and disease, offering accurate and reliable data for further investigation and potential therapeutic development.
Product Name:
Mouse C3 (Complement 3) ELISA Kit
SKU:
MOES00893
Target:
Mouse C3 (Complement 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3. You can calculate the concentration of Mouse C3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
99-112
95-108
Average (%)
99
106
102
1:4
Range (%)
88-102
87-97
87-102
Average (%)
95
92
93
1:8
Range (%)
89-104
88-98
87-102
Average (%)
96
93
93
1:16
Range (%)
85-99
86-98
86-100
Average (%)
92
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
91-107
99
Cell culture media (n=5)
91-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.66
20.07
84.42
9.88
20.58
79.18
Standard deviation
0.74
1.14
3.29
0.64
1.2
2.99
C V (%)
6.94
5.68
3.9
6.48
5.83
3.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C3 in samples. No significant cross-reactivity or interference between Mouse C3 and analogues was observed.
Leavy, A., et al.
Investigating Pharmacological Inhibition of the NLRP3 Inflammasome Acutely and Chronically Post Hypoxia in a Moderate Model of Hypoxic-Ischaemic Encephalopathy
