The Mouse C4 (Complement Component 4) ELISA Kit is a highly reliable assay designed for the precise and quantitative measurement of C4 levels in a variety of mouse biological samples. Complement Component 4 is an essential protein involved in the complement system, playing a pivotal role in immune responses, inflammation, and clearance of pathogens from the body. Ensuring exceptional sensitivity and specificity, our Mouse C4 ELISA Kit delivers accurate and reproducible results, providing researchers with valuable insights into the role of C4 in immune modulation and disease mechanisms.
Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an excellent choice for advancing research in the fields of immunology and inflammation.
Product Name:
Mouse C4 (Complement Component 4) ELISA Kit
SKU:
AEES00215
Target:
Mouse C4 (Complement Component 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C4. You can calculate the concentration of Mouse C4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-95
95-102
92-103
Average (%)
92
98
100
1:4
Range (%)
88-99
93-101
92-105
Average (%)
96
96
99
1:8
Range (%)
95-108
86-97
91-100
Average (%)
102
93
97
1:16
Range (%)
101-110
90-98
97-107
Average (%)
104
95
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
97-107
101
Cell culture media (n=5)
100-108
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.17
120.98
473.35
43.16
117.19
464.94
Standard deviation
2.03
5.86
23.43
2.49
5.46
25.48
C V (%)
5.05
4.84
4.95
5.77
4.66
5.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C4 in samples. No significant cross-reactivity or interference between Mouse C4 and analogues was observed.
