The Mouse CAM (Calmodulin) CLIA Kit is specifically designed for the quantitative measurement of calmodulin levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and precision, guaranteeing accurate and consistent results for various research purposes.Calmodulin is a key regulatory protein that plays a vital role in calcium signaling and cellular processes such as muscle contraction, cell division, and neurotransmitter release. Dysregulation of calmodulin has been linked to various diseases including cardiovascular disorders, neurological conditions, and cancer, highlighting its significance as a biomarker for understanding disease mechanisms and developing targeted therapies.
The Mouse CAM CLIA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of calmodulin in physiological and pathological processes, offering valuable insights into disease mechanisms and potential therapeutic interventions.
Product Name:
Mouse CAM (Calmodulin) CLIA Kit
SKU:
MOES00324
Target:
Mouse CAM (Calmodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CAM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CAM. You can calculate the concentration of Mouse CAM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
101-117
88-103
Average (%)
103
109
94
1:4
Range (%)
97-114
100-114
101-117
Average (%)
105
106
109
1:8
Range (%)
90-102
88-104
90-101
Average (%)
95
95
96
1:16
Range (%)
96-108
104-120
96-110
Average (%)
103
110
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.23
236.49
769.41
112.01
250.48
716.66
Standard deviation
9.18
20.43
76.4
14.43
29.43
68.37
C V (%)
8.48
8.64
9.93
12.88
11.75
9.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CAM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CAM in samples. No significant cross-reactivity or interference between Mouse CAM and analogues was observed.
