The Mouse Caspase-3 (CASP3) CLIA Kit is specifically designed for the quantitative measurement of Caspase-3 levels in mouse serum, plasma, and tissue homogenates. This kit provides highly sensitive and accurate results, allowing researchers to confidently study apoptosis and cell death processes in mouse models.Caspase-3 is a key player in the execution phase of apoptosis, a tightly regulated process that eliminates damaged or unwanted cells. Dysregulation of Caspase-3 activity has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions, highlighting its importance as a biomarker and therapeutic target.
With its user-friendly protocol and reliable performance, the Mouse Caspase-3 CLIA Kit is an indispensable tool for investigating the role of Caspase-3 in physiological and pathological processes in mouse models. Trust in this kit for robust and reproducible data to advance your research in apoptosis-related pathways.
Product Name:
Mouse CASP3 (Caspase 3) CLIA Kit
SKU:
MOES00151
Target:
Mouse CASP3 (Caspase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CASP3. You can calculate the concentration of Mouse CASP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
90-104
97-109
Average (%)
94
95
103
1:4
Range (%)
99-112
102-115
87-98
Average (%)
106
109
92
1:8
Range (%)
100-116
92-106
92-108
Average (%)
108
100
99
1:16
Range (%)
91-106
99-117
86-100
Average (%)
98
107
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
98-111
103
Cell culture media (n=5)
96-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.41
182.66
989.75
100.43
172.59
907.84
Standard deviation
12.79
18.85
71.36
13.03
20.07
54.74
C V (%)
12.25
10.32
7.21
12.97
11.63
6.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CASP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP3 in samples. No significant cross-reactivity or interference between Mouse CASP3 and analogues was observed.
