The Mouse CC17 Clara Cell Protein CLIA Kit is specifically designed for the precise measurement of CC17 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it suitable for various research purposes.CC17, also known as Clara Cell 17, plays a vital role in lung health and respiratory function. It is involved in maintaining lung integrity, promoting tissue repair, and protecting against respiratory diseases.
By measuring CC17 levels, researchers can better understand lung function and assess the impact of various treatments on respiratory health.Whether studying lung diseases, respiratory disorders, or overall lung function, the Mouse CC17 Clara Cell Protein CLIA Kit offers a valuable tool for researchers seeking to unravel the complexities of lung biology and explore potential therapeutic interventions.
Product Name:
Mouse CC17 (Clara Cell Protein) CLIA Kit
SKU:
MOES00174
Target:
Mouse CC17 (Clara Cell Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CC17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CC17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CC17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CC17. You can calculate the concentration of Mouse CC17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
101-112
97-111
Average (%)
109
107
103
1:4
Range (%)
90-107
86-98
88-104
Average (%)
98
92
95
1:8
Range (%)
86-97
99-114
98-113
Average (%)
91
105
103
1:16
Range (%)
92-106
85-99
88-101
Average (%)
98
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
98-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.64
233.77
909.59
85.41
214.46
988.06
Standard deviation
10.94
27.7
70.49
6.97
20.63
82.11
C V (%)
11.94
11.85
7.75
8.16
9.62
8.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CC17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CC17 in samples. No significant cross-reactivity or interference between Mouse CC17 and analogues was observed.
