The Mouse CCKAR (Cholecystokinin A Receptor) CLIA Kit is designed for the precise measurement of CCKAR levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers high accuracy and specificity, ensuring dependable and consistent results for a variety of research purposes.The CCKAR receptor is a key component in regulating gastrointestinal functions and appetite control. Dysregulation of this receptor is associated with various digestive disorders and metabolic conditions.
By accurately measuring CCKAR levels, researchers can gain valuable insights into these conditions and explore potential therapeutic interventions.Overall, the Mouse CCKAR CLIA Kit is a valuable tool for studying the role of CCKAR in physiological and pathological processes, providing researchers with reliable data to advance their research goals.
Product Name:
Mouse CCKAR (Cholecystokinin A Receptor) CLIA Kit
SKU:
MOES00169
Target:
Mouse CCKAR (Cholecystokinin A Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCKAR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCKAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCKAR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CCKAR. You can calculate the concentration of Mouse CCKAR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
98-113
86-97
Average (%)
103
105
91
1:4
Range (%)
89-107
85-95
93-107
Average (%)
97
90
99
1:8
Range (%)
93-108
92-106
86-101
Average (%)
101
98
93
1:16
Range (%)
100-117
102-116
88-98
Average (%)
107
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.96
310.79
940.32
95.07
309.9
908.51
Standard deviation
11.88
22.1
98.73
7.64
23.55
76.59
C V (%)
12.25
7.11
10.5
8.04
7.6
8.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CCKAR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CCKAR in samples. No significant cross-reactivity or interference between Mouse CCKAR and analogues was observed.
