The Mouse CD162 Monoclonal Antibody (PERCP-Cyanine5.5 Conjugated, 4RA10) is a specialized tool for researchers studying CD162, a cell surface glycoprotein involved in immune cell adhesion and migration. This antibody, designed for use in flow cytometry applications, has been meticulously validated for its specificity and sensitivity in detecting CD162 expression in mouse samples.CD162, also known as P-selectin glycoprotein ligand-1 (PSGL-1), plays a key role in mediating interactions between immune cells and endothelial cells during inflammatory responses. Its involvement in leukocyte rolling and adhesion processes makes it a crucial target for investigation in immunology studies, particularly those focused on inflammatory disorders, infectious diseases, and immune cell trafficking.
By utilizing the Mouse CD162 Monoclonal Antibody, researchers can efficiently analyze the expression of CD162 on various immune cell populations, gaining insights into its function and potential therapeutic applications. This antibody's high specificity and fluorescence properties make it an essential tool for advancing our understanding of immune cell behavior and developing novel approaches to modulate immune responses.
P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG1, κ
Isotype Control:
PerCP/Cyanine5.5 Rat IgG1, κ Isotype Control[HRPN]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
Q62170
Gene ID:
20345
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.
![PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0845)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/237410/618122/percpcyanine5.5-anti-mouse-cd162-antibody-4ra10-agel0845__47137.1732237915.jpg?c=2?imbypass=on "PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0845)")
![PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0845)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/237410/618122/percpcyanine5.5-anti-mouse-cd162-antibody-4ra10-agel0845__47137.1732237915.jpg?c=2 "PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0845)")
![PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0853)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/21384/606825/percpcyanine5.5-anti-mouse-cd162-antibody-4ra10-agel0853__58864.1707499266.jpg?c=2 "PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0853)")
![PE/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0852)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/21383/605925/pecyanine5.5-anti-mouse-cd162-antibody-4ra10-agel0852__25612.1707496394.jpg?c=2 "PE/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0852)")
![PE/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0844)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/21375/606759/pecyanine5.5-anti-mouse-cd162-antibody-4ra10-agel0844__68447.1707499030.jpg?c=2 "PE/Cyanine5.5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0844)")
![APC Anti-Mouse CD162 Antibody [4RA10] (AGEL0841)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/21372/606153/apc-anti-mouse-cd162-antibody-4ra10-agel0841__07763.1707497106.jpg?c=2 "APC Anti-Mouse CD162 Antibody [4RA10] (AGEL0841)")
![Purified Anti-Mouse CD162 Antibody [4RA10] (AGEL0159)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/20105/606518/anti-mouse-cd162-antibody-4ra10-agel0159__53640.1707498291.jpg?c=2 "Purified Anti-Mouse CD162 Antibody [4RA10] (AGEL0159)")