The Mouse CD162 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated, 4RA10) is a versatile tool for researchers interested in studying CD162, a cell surface molecule with important implications in immune responses. This monoclonal antibody, conjugated with PerCP/Cyanine5.5, offers high specificity and sensitivity for detecting CD162 in mouse samples. It has been rigorously validated for various applications, including flow cytometry, immunofluorescence, and immunohistochemistry.CD162, also known as selectin P ligand (PSGL-1), plays a crucial role in mediating leukocyte rolling and recruitment during inflammation and immune responses. Its interaction with selectins is essential for proper immune cell trafficking in various physiological and pathological conditions.
By targeting CD162, researchers can gain insights into the mechanisms underlying immune cell adhesion and migration, which are pivotal processes in host defense, inflammatory disorders, and cancer progression.Whether investigating the role of CD162 in leukocyte function, studying inflammation dynamics, or exploring therapeutic interventions targeting immune cell adhesion, the Mouse CD162 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated, 4RA10) is a valuable asset for advancing research in immunology, inflammation, and related fields.
P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG1, κ
Isotype Control:
PerCP/Cyanine5.5 Rat IgG1, κ Isotype Control[HRPN]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
Q62170
Gene ID:
20345
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.
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