The Mouse CD8a Monoclonal Antibody (53-6.7) is a valuable tool for researchers investigating immune responses and T cell biology in murine models. This monoclonal antibody, designated AGEL0378, specifically targets CD8a, a critical cell surface marker expressed on cytotoxic T cells.When studying immune responses in mice, the CD8a marker serves as a key indicator of cytotoxic T cell activation and function. This antibody has been meticulously developed to recognize and bind to CD8a, facilitating its detection and analysis in various experimental settings. Validated for use in techniques such as flow cytometry, immunohistochemistry, and immunofluorescence, this antibody enables precise visualization and characterization of CD8a-expressing cells in tissues and cell cultures.By utilizing the Mouse CD8a Monoclonal Antibody (53-6.
7), researchers can gain deeper insights into the role of CD8a-positive T cells in host defense, immune surveillance, autoimmune diseases, and tumor immunity. This antibody is a critical asset for studies aiming to decipher the complex interactions and functions of CD8a-expressing T cells in the context of infectious diseases, cancer immunotherapy, and immune system dysregulation.With its high specificity, reliability, and compatibility with various research applications, the Mouse CD8a Monoclonal Antibody (53-6.7) from AssayGenie empowers scientists to advance their understanding of immune responses and T cell-mediated immunity in mouse models, contributing to the development of innovative therapeutic strategies and interventions in immunology and cancer research.
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
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